Some how I feel this has come up
So I just set up the new fish tank for both my wife and daughter and they like glowfish. I'm sure a few already see where this is going.
I know the gene is from a jellyfish and injected into the fish egg. Then the offspring of the injected fish has the gene to glow and will glow.
Now the obvious question. Can this be adapted to a mammal such as chicken or other animal. I'm clearly thinking human as well but that time line to see it would take AWHILE.
I know the gene is from a jellyfish and injected into the fish egg. Then the offspring of the injected fish has the gene to glow and will glow.
Now the obvious question. Can this be adapted to a mammal such as chicken or other animal. I'm clearly thinking human as well but that time line to see it would take AWHILE.
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It would still be pretty cool having a few baby glowing crested geckos
A lot of the work that was done with with fish and other mammals showed from the offspring of the injected parents.
Wouldn't the bacteria approach not carry on into offspring? That I feel would be a more expensive route to take if it isn't inherited.
I feel like your approach would be simpler but is for an already hatched critter vs one in an egg developing.
Sorry for my poor knowledge on biology it's far from my strong suit.
From what I'm gathering I'd be changing the parent geckos bacteria (still not 100% understand that part but that's why they made Google;) ) and the observable change would be in offspring assuming it all took.
I believe bacteria GFP is actually cheaper then the other option for injecting embryos. I'd have to look again.
On a side note how feasible is it to harvest the protein from a glow fish and make that usable?
I'm hoping some one with more biology back ground could chime in with some 2 cents on how it could even be accomplished.
@dr_allcome do you know what it would require to simply get to the point of being capable of doing this with the bacteria? I mean equipment wise.
@EmberErikson That's actually a lot harder. You'd need a viral vector to do that which has it own host of issues and also all of the cancer is a legitimate issue. If you just want to glow temporarily, I was talking about it in another thread, you could use something like a liposome to deliver the plasmid to your cells in a local area. So long as it doesn't try an integrate and doesn't just get chewed up by your immune system it should cause the cells affected to express the protein until they die. When they do the plasmid will be destroyed and you'll stop glowing. I'd stick with something simple like gfp. It's still difficult but less than a virus. Again, own host of issues, but that's a topic for another day. LINK to other thread
Also, if ya'll want to learn all this kind of stuff, might i suggest exosphere? LINK
Thank you both for that massive amount of info and I'll definitely look at exosphere.
Pglo:
With that kit it would alow me to strictly modify bacteria? This would in fact alow me to do as @dr_allcome mentioned and infect the animal (lizard) with the affected bacteria (hopefully) not harming it in the process assuming I used bacteria from the lizard (natural flora)?
Glowing:
Fully understand the glowing under black Light. Many geckos are often kept under black Light in general at night because it doesn't affect their vision negatively. So a black Light based glow is acceptable.
Guns:
Would this be needed (never thought I'd say that under the word guns) if you were to go the "roundabout" rt with bacteria still?
Exosphere:
Some how I missed your video on it and the last link you posted to it was back to this thread. I did get to the site though. Unfortunately I am stuck on the east coast without the ability to travel and spend that amount of time out of work. :(
I feel like I missed something but I think this covers my foggy brain thoughts. I'll have to look at the posts again after some sleep.
Side note the most biology I know is Pennetta squares which I used mostly for breeding geckos. I'll try and keep up with what's posted on this very weak subject of mine.
I suppose the next thing I should probably do it learn about a gene gun and the simpler genetic engineering. That kit is a very welcoming price to get though which is nice.
I did a look over on the gene guns and it seems very simplistic in design. I'm hoping I'm wrong because I'm sure it costs a good amount of money to buy one.
1)force builds up behind disk
2)disc breaks and air flow travels through "barrel"
3)hits nano particles/DNA?
4)force pushes particles into cells of plant/animal
A wiki searched indicated that an air soft gun was originally used and then refined.....
I've been looking at glass ware and such to try and start up having equipment to do more then just electronics.
EDIT: forgot to mention I was just watching a video of Pic2 being made and was going to do that route for making one. I just wasn't sure it would function. Again it seemed to simple.