dokclaw

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dokclaw
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  • @thegreyknight if you're familiar with the R language at all, there is an enormous community of bioinformaticians who've made a large number of functions and tools to allow you to quickly access databases. (Here's a link to a quick google search res…
  • How about UV sterilization followed by ethanol rinsing? This is usually enough to keep bugs out of tissue culture in the lab... Is this something you've tried?
  • Have you thought about epigenetic targeting? If you could increase the methylation of the myostatin gene, you could really reduce its transcription. I think if you targeted the transcription factors that regulate myostatin expression, you'd end up w…
  • See my comment in the electrotransfer thread. 
  • I know that the ethos of this forum is one of DIY science and trying things out to see if they work, but when it comes to genetic modification of ourselves or our microbiomes I am incredibly cautious about it, and I don't think that anyone will ever…
  • You can overdose on Vitamin A though, and Vitamin C IIRC...
  • That's how insulin is made commercially; in bacteria in the lab. Sticking those bacteria in the gut of an animal is not going to work to treat diabetes, otherwise it would have been tried.
  • I shall enter with my customary wet blanket. *Drip, drip*. Genes that encode resistance to arsenic in bacteria will allow the bacteria to survive arsenic poisoning, not the host.  Additionally, the introduction of foreign bacteria or genes into th…
  • https://www.kickstarter.com/projects/chaibio/open-qpcr-dna-diagnostics-for-everyone?ref=NewsNov0614&utm_campaign=Nov+06&utm_medium=email&utm_source=newsletter
  • It looks cool, but then it's supposed to given that it's just art. It's commentary on how hungry we are for new ways to power our world; I don't think it's supposed to be an actual power source... At least that's how I read it. I was very surprised…
  • Commercially available electroporators are generally either cuvette-based, or have hand/clamp held electrodes. The area that is actually electroporated is really, really small. Maybe 5mm2 MAX with the hand-helds (and I couldn't say what the electric…
  • What's the goal here? I ask because I work in a research lab where we routinely electroporate chick embryos, and it being "electrically simple" doesn't mean that it isn't otherwise tricky. I might be able to provide some insight, or at lea…
  • IIRC, Tim took out the Circadia earlier this year due to complications. My word is by no means gospel on this matter though, so you should check around. He's on this forum, though I cannot remember his username.
  • I just came across a link to the article about sweat-powered batteries, and was about to post it, then realised that kjwx had already done so! The cathode is platinum, which (currently) costs $1460 per oz. The output is 70µW per cm2. I'm pretty su…
  • From what I remember, that leaf is essentially a support scaffold for chloroplasts, which you still have to harvest from plants, then install into the body (in sufficient density) without an immune response.
  • SfM? I would have thought that corporate (i.e. for-profit) pricing would be higher... I didn't want to bother with getting a quote for these things, so $2000 was based on the price of the helium "compressor" kit; as you say, the gun itself…
    in Gene Gun Comment by dokclaw August 2014
  • $600 for the particles (gene carriers), well over $2000 for the rest of the kit (inferred). This is pricing for academic/pharmaceutical research...
    in Gene Gun Comment by dokclaw August 2014
  • Regarding the density of Merkel cells in various areas, I think that the textbook is stating that the ratio of Merkel cells to sensory neurons is 50:1 in the forearm, and 5:1 in the fingertips, meaning that the resolution is higher in the fingertips…
  • Merkel cells seem like a good bet; I was (stunningly) unaware of their existence! It's nice that you don't have to target neurons, because you almost certainly would get crossover. Both those papers look good; I was initially concerned that they w…
  • I'm no use as an electrical engineer, but I came across some work from this guy's lab where they were implanting chips in mice, and using inductive coupling to power the device. Here's a link to his lab's webpage: https://engineering.purdue.edu/BME…
  • I have an academic interest in sensory neurons, and unless I'm very much mistaken, you won't be able to target specific types of sensory neuron with perfect accuracy; there will inevitably be some crossover between modalities. The worry here would b…