glims

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glims
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  • How exactly does being a biohacker mean that killing half a dozen monkeys would be good numbers? The numbers suck no matter who presents them. And one doesn't get a special pass for crappy numbers and monkey killing just because one is a "biohacker". Why didn't it work? It was unsafe. They used money to rush the process.…
  • Clostridium sporogenes is already present in the guts of some of the population, so that sounds reasonable. However, a lot of gut biome stuff deals with lifestyle changes. C spor is in the soil which means we all have been exposed. You would want to look into the things that would keep it around, not just adding it. also,…
  • Oh, hmmm, I missed this. Hopefully you are still compiling. I personally would not at this time take aav-htert, but I have helped people develop it. I have worked with various groups, including consulting for Parrish right after she got her first injections. We both lived in Seattle at the time. I know some stuff that…
  • Not really interested in having a mentally unbalanced, power hungry, group of wealthy people start calling them selves shamans because they drink kale juice. It was interesting to listen to, if only a bit disturbing.
  • Got a list of things to discuss here. The first and foremost is that this is not a good idea for people to "achieve a goal" in being trans. This implies that I can edit the gay out of someone as well, and unless anyone is ready to tackle supporting that concept, we should all just curb the enthusiasm. If this in theory is…
  • Wouldn't you need to do this alteration in the somatic cells? What vector did you use? AAV holds up to 4.5k or so, lenti caps out at around 10k. You've got a chunk of SV40 in there but SV40 only holds up to 5k as well.
  • @ManiacBrainiac I have been working on this project for the last ...3(?) years. What you want to do is create a BAC, bacterial artificial chromosome. And you can already order the virus with the knock out. Just remember that you need a -80C freezer for viral storage, and liquid nitrogen for mammalian cell work.…
  • @Pritchdex @Midoriya "Something you might be interested in -- the U.S. Army did an experiment with high doses of vitamin A2 that extended the vision of test subjects by about 20nm. It's not much, but given that you can get rid of your (very minimal) IR vision whenever you want, it seems like a better (but less effective)…
  • Can you elaborate on your initial post here? Comments have already run the gamut from protocol repository to zine. None of which are actually peer reviewed in as much as there isn't in this community a pre established infrastructure of collaboration and verification of work. The very nature of the grinder ethos lends…
  • reposted from where I put it in the codes of conduct vs here at code of ethics. My apologies. So many shiny new rule sets. What are the methods for self policing? Will we start banning people who start with "so i don't have lido so I'm using ice..." and does that violate being inclusive? What if that person just needs…
  • Yep. Saw them both in the same space, clicked on the wrong one. My bad. Carry on. EDIT: removed comments as they are not on topic.
  • I'm gonna regret this, but it's all hypothetical, right? Swim should probably start altering his place in life, not altering his brain chemistry with different chemicals than the aforementioned happy pills and potentially harming himself. Swim seems to have given you some conflicting information. He is either dissatisfied…
  • The answer is the egg. You modify the egg. But also, yeah, one step at a time. Not gatekeeping here, but if you don't know what to modify, probably shouldn't modify.
  • http://libgen.io/search.php?req=crispr&lg_topic=libgen&open=0&view=simple&res=25&phrase=1&column=def Start with the addgene document and work your way up.
  • When you're brainstorming a project like this, it's always important to ask yourself, why would the bacteria care about what you are doing? Then being extremeophiles isn't a bug, it's a feature, for them. Those bugs are features! Puns! Anyway. We only use extremeopgiles in really weird cases where you are engineering…
  • Biology is weird that way. Big results require big alterations. Just to be clear, nobody stab their eyes please. I looked into possibly using the delivery method we used for the ce6. It doesn't get to the right location.
  • Yeah, we're still here. We're just lazy turnips. Also, I commented in this thread the same day you said you don't see me around anymore. Geez, you stop yelling at people and they go soft. My vote is : replication ease - high application ease - way low and probably stupid to do at home potential side effects - way to early…
  • I mean, that's some pretty good data there. And it's direct. It's not like they were like, hey we did this thing and so we think that this other thing will happen. They tested it well, too. I'd wait a little bit longer for negative side effects to show up.
  • Can you be more specific about what you mean by 'info about crispr'?
  • Do they not have what you're looking for or is there another reason you're looking elsewhere?
  • I feel like we've gotten a bit off topic. Given the new information, could you redefine and reoutline your plan?
  • Fresh spideys are incoming, along with more supplies.
  • @ThermalWinter Are you asking for the papers that we are using to back our projects? I can put those together and dump them into the dropbox. All the projects? As for the plant project, that is off topic for this thread. If you want to start another thread or pm me, I'm totally down to talk more about it. I've been slow…
  • @RealityWizard "Research in a non scientific manner"? What does that even mean? I work in a lab. You can't do lab work without using the scientific method. Is it a fun lab? I guess so. I'm not a huge fan of how you delineate between projects. We do all of the options you outlined. That's kind of the point of having your…
  • For sure. I'm not saying it's not doable. Just that the hurdle is a bit high atm
  • Yeah, mammalian work is easy but expensive. I'm not suffering from anything except a lack of sleep at the moment. I just kinda started working on this for a friend and it became a thing. Chironex outlined it very well. He was there when we put together the lab. As he mentioned, the thing you are attacking from a software…
  • I'm working with HSV right now. What are you doing?
  • @Moonman0922 no, no, that's @chrionex. He helped me set up a lab in the US and we did some projects together. He's back in Canada now with a new lab of his own.
  • Have you already looked into washi paper making techniques. Physical application of materials is so crucial in material science stuff The process itself creates a really durable paper.
  • @Verta the vectors are a 3part BAC. I store them in the fridge/freezer/-80degC/liquid nitrogen, depending on what I'm using. In a tube.