chironex
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- chironex
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Comments
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Ok so this thread has gotten beyond tiring at this point. We literally just outlined how the virus is treated, and thus far you've just chosen to ignore the actual experts and continue responding with essentilly random biology buzzwords while you're…
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Not yet. Waiting on more supplies, building equipment, testing and restesting protocols. Nothing exciting. This is gonna be a slow burner project with lots of fits and starts. The hurdles we have to clear are immense and currently material is hard t…
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you'll just crush the tissue between the magnetizer and your magnet most likely. A magnetizer is just a giant electromagnet. You end up applying as much field as you can to align the magnets electrons spin. Which in this case results in crushed skin…
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Ima just say that if you're at the "building a centrifuge" stage, you're about 5 years from doing anything. If you're not spending 50 bucks on a fuge, you're not even close to the 50k+ it'll take you to actually do anything with a virus safely AND h…
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Ya my new lab has taken me months to put together. Mine is based out of a larger community hackerspace and I managed to aquire one small corner to build the lab in. Whole room is 12' x 7'. Paid for everything out of pocket, which is also why I've be…
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Obviously nonsense is obvious. Lay off the drugs and next time show up with real data or gtfo. Thread closed.
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Updated 2019/01/27
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New video is up. Not strictly spider silk stuff but its what I've been keeping busy with while I wait for fresh spiders to arrive.
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New video and I discuss the issues with the spiders:
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I've got a PRP experiment partially filmed we started a year ago. Bout to restart that series of experiments, just waiting on some new items to arrive (blood draw kit mostly). I agree, fuck the kits. All you need is vacutainers with acid citrate dex…
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Yup! a spec would cost 400, we built one for like 40 bucks. An ultrasonic homogenizer would cost over 1k. And a PCR machine with that capability costs hundreds as well. Built the whole set for less than 100 total. Still gonna keep tinkering with all…
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@misslitty ya it's getting annoying I'll admit. We're planning an easier experiment that's just for fun so we can get at least 1 genetic engineering win to keep us going. I've been keeping busy in the mean time honestly. Been doing some machining a…
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Womp womp. Negatory. Gonna source some fresher spiders and order more primers and try again
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@misslitty thats the one! We're running it today hopefully. will post results when they come back. Also will post a less ugly gel from the extraction
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@misslitty that's the plan! just need to get some first. still haven't run the pcr yet though. if that comes back positive then we won't need to.
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Test results came back. Got lots of DNA, but when we ran it on a gel it all looks sheared to hell. Still gonna try PCR, but we're starting to suspect that the preservation method of our source spiders isn't good and the DNA degraded. The ethanol pre…
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Ran another extraction today. At this point I just assume it's garbage so I'll be excited when it finally does work. Used a professional kit this time. God damn was that a long procedure. 27 steps, some loop back, and some have up to a 4 hour incuba…
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Update time. Tried more extractions. None worked properly. Showed nice peaks on a nanodrop, but garbage on a gel. Ordered more spiders, they just arrived, as well as a proper DNA extraction kit. So gonna give that a go hopefully this week. …
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They sell parylene only magnets last i looked, and they'll do the double coat if you ask for it specifically.
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the issue is that ya'll are getting the TiN only magnets. send them a message and see if they're double coat a batch for you. TiN and then parylene. Fixes the failure rate by plugging any holes in the TiN
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"don't need to use anything sketchy like glyphosphate" wants to use spider venom. LOL As to making it, looks super easy tbh. It's 37aa peptide which means it'd take ~110bp of DNA. That's honestly nothing. You can get that made by a primer synthe…
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Doesn't matter. You're doing surgery, on yourself. Follow the fucking protocol, or don't do it. Isn't negotiable.
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K im only gonna say this once. If you can't get lidocaine, you shouldn't be doing implant surgery. Ice is not a viable replacement. For fucks sake, get some lido. I honestly do not understand why this is even a topic that keeps coming up. You are do…
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Some updates. We tried a weird PCR reaction directly on a piece of spider tissue but it didn't work. HOWEVER another run we did on a reaction that should have worked, didn't. Which means my machine might just be crap. So we're gonna run a reaction t…
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If you don't want anyone filming anything, get a bunch of IR lights, string them up all over the place and make them strobe at about a couple times per second and get some speakers and play a nice loud tone at about 30khz. Nothing will pick up anyth…
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Latest update and part of the series!
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Secondary update, it's becoming very obvious why our DNA extraction failed. I mean we knew we were using the wrong kit to start with, but proper insect extraction calls for incubation in lysis buffer overnight at 56c rather than the 4 minutes at rt …
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Did someone order a big load of fuck all? We think the issue was our terrible DNA extraction. Gonna redo that with the new extraction procedure, then try again once we've confirmed we have lots of genomic DNA to work with. If that fails we'll re…
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The three that worked are all from the same thing just at three different annealing temps. Though the cycle was run with a gradient decreasing in temp each cycle by 1.2 degrees. So in order it's 72, 68, and 65c. But ya paper based didn't work but…
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