BMedSci

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BMedSci
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  • Ok, so let's say I want to make this from scratch I think this is my shopping list. 1: Plasmid that is big enough2: Restriction enzymes that are specific for sites on the plasmid3: Gene which has specific sticky ends for the restriction enzymes4: DNA ligase5: E.Coli in which I can multiply the plasmid6; Sterile plates7:…
  • I think that the best way to go is probably GFP as a prototype since its been engineered into mice without killing them it seems like a fairly safe starting point in terms of not wanting to die and if it worked in mice there might be a chance that it would work for all mammals. Worst case I could email the lab that did it…
  • Hey again, I've come across something in my studies that could be a possible solution. It kinda seems very simplistic but apparently the application of naked DNA actually works to deliver DNA to topical areas (but that using something called microemulsion delivery system, which I think is the zwitterion method you're…
  • Nice, I'll do some serious research into this in my spare time (and probably mostly after exam season). So far I'm thinking this -Protein=GFP (though might look into others/modification of GFP to stop it being degraded)-Delivery=liposome This is where I don't know what would be best: -Nucleic Acid=pure RNA because I don't…
  • Just an idea but why does it have to be visible? Could you possibly make it into something you can read by touch (maybe with little pins that spell out the time) that you could feel through your skin with your finger and thus save energy on making the thing emit light through the skin.
  • Yeah, I've been told that a few of these ideas had been floated around. I don't know if this has been suggested but I think that inserting RNA inside a liposome packaging would be a good proof of concept if you have any idea how to do such a thing? On another note, this would more than certainly cause an immune reaction…