misslitty
Comments
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Thank you both for your input! I have been away for quite awhile, I'll check out both.
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If the fibrous tissue remains, and becomes a nuisance, I'd suggest Kenalog. I injected a different grinder who had a gnarly granuloma following a magnet removal, and it definitely helped it shrink. Wishing you a speedy recovery!
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One possible reason is that the ring finger is substantially longer than the pinky, and would rub against it less, causing less irritation. The middle finger is typically longer than the ring, however, and would result in more contact and likely more irritation.
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This is my shit, I've been working with platelet rich plasma in my research for three years now and have also been helping out a colleague using in for cosmetology applications. The pre-fab kits are an absolute rip off- all you need is the proper anticoagulant and a centrifuge. I've always wondered about how much the…
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God I have so much brain envy, there are no words. I'm assuming it's cheaper to build all of those yourself than to find one someone is trying to get rid of? In any case, that's beyond cool, and having full PCR temp control will be nice! Really hoping the new primers solve your issue, it would be a little insane to build…
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): Can't imagine your frustration. Keep on keeping on, you got this!
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This would be the PCR w shorter primers? Hope it works out!
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@chironex Are you gunna smash some fresh spideys or what?
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Thank you all for your input! I made sure to not implant it too close to any major arteries or veins, but it would probably be smart to at least know where it is. @RaelAdir that's quite a distance to migrate! Did that happen during the initial implant healing time? Also, just for the sake of information, has anyone ever…
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What's in what lane? And does that mean the paper-based extraction didn't work? In any case, very exciting!!
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Also a great paper! I think that's the big problem with any implant- there is no way so far to mitigate the foreign body response
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The paper you linked concluded: "We feel that any material which allows close contact of living cells at its surface, which does not contain leachables which produce inflammation and which does not prevent growth and division of cells in culture, can be considered biocompatible. Such materials are the bioglasses used in…
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@chironex Haha live and learn, I suppose. I suspect having separate DNTPs and all will make your life a lot easier. Sending good research and spidey vibes- keep updating us on this please!
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@chironex Oh I suppose that may have backfired a bit. Is the wax at least more affordable? And that is so cool! Have you played around with manganese before? Also, I'm sorry but I don't have any of the plasmid, worked on project that in a different country. I'm so stoked for this spider silk experiment, though, and can't…
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@chironex * Lol that's hilarious. Did they properly compensate for the monumental fuck up? * Never heard of wax before, is it similar to DNTPs? * A little unrelated, but at the end of your video you mentioned trying out new colors. I found out during my work that mCherry's beta barrels actually result in a more stable…
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Almost died, but the conference itself was good! I don't know if there was anything super pertinent to biohacking, but there was an interesting talk on biobots and how in examining the actin attachment, a researcher may have found an application for sickle cell disease. Also a lot of very cool lab-on-a-chip and organoid…
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That definitely sounds like a primer issue to me. Could it be that, as you mentioned, the sequence you received didn't match up with what it was supposed to be and primers didn't actually cut anything?
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They aren't the easiest to work with, especially for such a big protein, but I'm sure you'll get it working! When are you planning to transfect? Also, side note, but you should really consider becoming a professor.
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Excited for updates on this! Fingers crossed the yeast doesn't give you too many problems.
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I'll let you know if there's anything wildly interesting!
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* In addition to loss of strength, there's a chance that once the neodymium is exposed, your body will reject it. It will become sore and inflamed, and the magnet will gradually migrate to the surface. * Not exactly what you asked, but if you have access to a stereo microscope I would suggest also visually inspecting the…
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Are you proposing designing a MagR compatible with human blood?
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I have a bit of experience with microencapsulation using silica, which may be comparable if the powder granules are fine enough. I'll try to hunt down my protocol- it was done in a lab but with access to a centrifuge it could easily be tried.
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What do you do in food service? Cook, server, host? Depending what you do, you may not have to take any time off of work. https://forum.biohack.me/index.php?p=/discussion/1791/first-magnet-implant I tracked healing once mine was implanted, since it seemed like there weren't many resources for that. I think a lot of it…
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Have you looked into the Utah Electrode Array? I know that Warwick has one, and my university is doing extensive research on them. They aren't exactly commercially available though.
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I'm a researcher in a biopolymers lab while I finish my biomedical engineering and chemistry studies
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E. coli is incredibly easy to work with, and so long as you prepared your media properly and under sterile conditions (are you adding antibiotics?) I think that would be fine!
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This isn't my area so apologies if these are stupid questions- 1. How noticeable is roid rage for any of you who regularly partake? I used to have this stalker who got violent and was a heavy steroid user, I'm curious how related that actually was. 2. Do you get any interesting food cravings, like with corticosteroids?
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Oh god that does look a little rough. When you say it was dark, you mean darker than the new M31s? And it does look like it'd been damaged, but you'd think TiN wouldn't be so fragile. I get paranoid that the coating on mine has ruptured and it's concerning that sometimes there are so few signs.
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@BirdMachine How bad was it when you pulled it? It felt normal other than the loss of magnetism?