Knockout of FOXL2 In Vivo?
A paper was published a few years ago (https://cell.com/fulltext/S0092-8674%2809%2901433-0) detailing a study where adult ovaries were transformed into testes via ablation of FOXL2 in mice.
I'm trans-masc and nonbinary, I have been dealing with extreme gender dysphoria my whole life. Hence, my motivation to knock out FOXL2.
My idea is to use SpCas9-HF1 to Knockout FOXL2 by creating nano-particles of the cas9 and gRNA in chitosan (chitosan has been shown to be a potential vector https://ncbi.nlm.nih.gov/pmc/articles/PMC4717060/?fbclid=IwAR0K7C8GounA9DZwkIRm8qSGMSKuo_uHsl8-2qd2fGjnIgn5SkPXddcODsY, although it is not as effective as AAV), then inject them into the blood stream.
Assuming I can source all the material, how likely is this to work?