I made a Crispr-Cas9 viral vector to knockout the DMRT1 gene in humans and had it manufactured

HelenahHelenah
edited May 22 in Genetic and biology based mods

A long time ago I made a Crispr-Cas9 viral vector to knockout the DMRT1 gene in humans and had it manufactured. Just wondering what the responses to this would be seems as I haven't dared tell anyone until recently due to legalities around all this. For those who don't know what this gene is responsible for, wikipedia explains it well: https://en.wikipedia.org/wiki/DMRT1

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  • glimsglims

    Wouldn't you need to do this alteration in the somatic cells? What vector did you use? AAV holds up to 4.5k or so, lenti caps out at around 10k. You've got a chunk of SV40 in there but SV40 only holds up to 5k as well.

  • HelenahHelenah

    @glims said:
    Wouldn't you need to do this alteration in the somatic cells? What vector did you use? AAV holds up to 4.5k or so, lenti caps out at around 10k. You've got a chunk of SV40 in there but SV40 only holds up to 5k as well.

    I took a big risk, it uses Lentivirus. If you want my honesty, I have no fear of death or the pain associated with it and I was willing to take risks.

    Based on tests, what I did worked. Please see attached file.

  • naisukhynaisukhy
    edited July 13

    Could you link the plasmid you made Helenah? How did you do this as well? Thanks in advance!

  • FrankFrank

    Interesting. What's your hoped for goal?

  • NotaSparrowNotaSparrow
    edited July 31
    I don't see many benefits for knocking out DMRT1 only a lot of problems . Is your goal to hurt people?
  • ArcMcNabbsArcMcNabbs

    This could be an incredible method of achieving a goal sex/gender in trans individuals. Absolutely incredible.

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