Looking for tips on cloning e coli for complete Myostatin knockout expirament in near future
I've been interested in Myostatin gene deletion for some time. e-coli is the carrier for the human Myostatin knockout Targeting crispr cas-9 plasmid I'll be using, however the dna concentration in the kit is not high enough. Dna replication can happen if I can grow a larger e-coli culture. Anyone know the exact procedure for growing e-coli in a controlled fashion? Once I grow a suitable amount of the bacteria I'll be using an endotoxin free plasmid dna isolation kit to isolate the human Myostatin knockout Targeting crispr cas-9 plasmid. Once isolated it will be combined with polyethylenimine in order to allow delivery into the cells. Any information on how to grow an e coli culture would be greatly appreciated. My observations results and any other questions I have will be posted here once I can begin this expirament.