Adding genes to zebrafish embryos, newbie, help plz
Hey everyone, I'm new here.
My name's Vince, I'm trying to add a gene to zebrafish embryos for a science fair project. I'm new to biohacking (like brand new) but I'm pretty familiar with the field of biology, I just get lost when it comes down to the nitty gritty technological side. I don't have the money to spend on microinjection equipment, so I was thinking electrofusion (if that's the correct terminology). I'm buying the gene (likely GFP) from an online supplier and I already have a group of spawning zebrafish. Does anyone know where I can find step by step instructions on how to do this and what equipment I will need (preferably as much DIY equip. as possible)? Even a link to information on the basics of this process so I can educate myself properly would be useful. I know this isn't like baking a cake, but with the right resources, I'm sure I could make myself competent.
Your guidance is very much appreciated!