Recombinant Virus Creation

Hello,

Does anyone know how to edit a virus using materials I can purchase to perform in my home?

Comments

  • Sure. Do you have an idea of how any of that works or is this more like a thought experiment?

  • AAVs and Lentiviruses are constructed by inserting 3 plasmids in mammal cells, then the cells produce the virus for extraction. I suppose you could edit the plasmids to therefore edit the produced viruses? I am not sure how do-able this is from home because most countries won't send you virus plasmids without a government approved lab.

  • Depends on your country and your paperwork on how hard it is to get them. It's more about your skill set and your tool access on if you can work with them. What is the hypothetical situation?

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