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learn how to use BLAST :https://blast.ncbi.nlm.nih.gov/Blast.cgi
and all the other pubmed resources
like genbank: https://www.ncbi.nlm.nih.gov/genbank/
the nucleotide database: https://www.ncbi.nlm.nih.gov/nuccore
the genome workbench: https://www.ncbi.nlm.nih.gov/tools/gbench/
and finally just get better at googling : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC109494/
I'm working with HSV right now. What are you doing?
Ima just say that if you're at the "building a centrifuge" stage, you're about 5 years from doing anything. If you're not spending 50 bucks on a fuge, you're not even close to the 50k+ it'll take you to actually do anything with a virus safely AND have it work.
Basically, there's 1 surface protein that masks it from the immune system. Break that and it becomes a glowing target and also can't enter cells. So if you introduce some live virus deficient in that gene, but grown in culture with the functional protein, you get basically 1 shot viruses. The first wave enter cells and make a ton of proteins, the immune system learns how to chew on it, but the virus can't spread once it escapes those first cells. Next time a normal HSV prticle shows up it gets eaten and that's the end of it.
Least that's the theory.
Also there's totally already a vaccine you can buy. Apparently the efficacy is a bit iffy because it's deactivated virus and not as good as the thing glims is tinkering with, but had some efficacy for some patients. May be worth a shot. https://rupharma.com/vitagerpavac/
A liquid nitrogen generator is fairly easy to make for under 800, I built one when I was 18. As for storing it you can get a dewar you can get a 35l for less than 200 from the hwbot.org forums if you are willing to do some waiting and driving. As for other stuff thst is out of my area of expertise but I am sure as the community grows it will become more accessible in the coming years.
@freedom_here it can take lots of time to do it properly if you are on a budget i did mine for under 600 but that took months of waiting on ebay and cl deals. You have to make sure your drying step and filter system is almost 100% or else you will get liquid o2 which is very bad. My first time around I got some and didn't notice until I light my torch and had a very startling experience, there was almost no liquid oxygen and it still was a bad time. You have to be careful and do it properly if not then I would suggest just buying it. I only pay 1$ per liter for my 50l dewar to be filed and I have heard of people getting it for as little as 50 cents.
I feel like we've gotten a bit off topic. Given the new information, could you redefine and reoutline your plan?
I plan on using DNA sequencing, and computer models, as well restrictive enzymes. I been considering getting a nano spore minion to observe crispr treatments results. But based on what you guys have said i will buy a bit more of my technology. I will continue to follow current gene editing techniques, but i believe crispr are the answer to find a cure for herpes.
Overall, For the next month I will be looking through human reference genome, and HSV 1 genome. I will be also doing more research into the minion nano spore technology. I will try to get a lab set up for next month as well. I will say this I will focus on find a cure for herpes, even if i have cut out all 77 proteins. So thank you for your help guys.
How is your progress going with the virus? Is there somewhere i can follow your research and progress.
Also what vector are you considering to deliver the treatment to the infected cells?
Sorry, for the many questions. I would also like to apologize for the long delay, in regards to my answers.
Ok so this thread has gotten beyond tiring at this point. We literally just outlined how the virus is treated, and thus far you've just chosen to ignore the actual experts and continue responding with essentilly random biology buzzwords while you're clearly not internalizing any of the advice people have given or info posted in this thread. If you'd like to follow the project head to scihouse.space, but it's biology, it's extremely slow, so constantly asking for updates is pointless. when there is progress, a post will be made. I'm closing this thread.