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Viral Editing?

ManiacBrainiac
ManiacBrainiac
First Anniversary First Post You Look Familiar Second Anniversary Third Anniversary Fourth Anniversary
January 2020
edited January 2020
in Genetic and biology based mods

Hi guys,

   I am a 4th year biotech UG with an interest in viruses. The herpes simplex ll virus cure was found by genetically removing a protein used to cloak the bodies immune response to prevent production of anti-bodies, the Gd2 protein.

I want to test this in mice, but I am not sure where to even begin?

Is there is a site I can order the modified virus in vaccine format for research? Subsequently, what if I wanted to test this on other viruses to removing cloaking mechanisms, how would I cut out a piece of virus RNA/DNA?

Tagged: 747, 1243, 1359

Comments

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  1. Pritchdex
    Pritchdex
    You Look Familiar First Post Paging Mr. F
    January 2020
    edited January 2020

    I would start with CRISPR. It's the most widespread/accessible method for cutting out specific genes from a segment of DNA that I am aware of. I'm no CRISPR expert, but it's probably worth looking into.

  2. glims
    glims
    First Anniversary Second Anniversary Third Anniversary Fourth Anniversary Fifth Anniversary First Post 10 Posts 100 Posts 500 Posts 1000 Posts You Look Familiar Welcome Committee Final Anniversary Paging Mr. F
    March 2020

    @ManiacBrainiac

    I have been working on this project for the last ...3(?) years. What you want to do is create a BAC, bacterial artificial chromosome. And you can already order the virus with the knock out. Just remember that you need a -80C freezer for viral storage, and liquid nitrogen for mammalian cell work.

    Fundamentals are crucial. Mammalian cell works starts in a flask. Not in mice. Mouse testing is ... well let's just say it';s more legal to stab yourself with something then it is to have an unlicensed mouse lab.

    DONT INJECT YOURSELF

    On that note, you would also need a way for the delta glyD viral particles to get into the cell. That means you need a secondary system set up to give the virus a one time pass that allows it to enter. This also requires a bsl2 lab and mammalian cells.

    If you want to learn more , ask away.

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