Is there such thing as a commercial, human Crispr gene knockout kit?
  • Arkcon10Arkcon10 August 2016
    As the title suggest's Im wondering if there's any commercial kits developed for the knockout of cells held within the human genome? E,g, a kit allowing for the infection of human cells to knockout (or perhaps add or alter) human genes? Are there even commercial kits for use on other animals like mice? or are we restricted to buying kits such as the, ODIN Kit specifically for bacterium? (and in a more specific case, the kit to add the green fluorescent protein to bacteria)? Would the same kit for the bacteria (or even the plant kit as i believe there is one), be able to be modified in such a way that it remove's or add's genetic information to a human cell? And even if there is a commercial human/animal cell kit, or a way to edit the bacterium/plant kits, how would one obtain/create their own genetic code to test within the target cell? To sum it all up im pretty much trying to see if its possible to conduct my own genetic experiments at home with the bare minimal lab equipment as i don't quite have access to a fancy lab, equipment nor even much time these days, yet i would still like to be able to do this   

    (also i understand that Human genetic editing is not exactly a "common science" in america, so i highly doubt there will be some form or any form of genomic kit for doing things such as this but i'd thought i'd ask anyways) 

    (TLDR: im asking if there's such thing as a human cell editing kit and if not, if there's a method of creating genetic code strands to be implemented into a pre existing kit) 
  • JordygordyJordygordy August 2016
    tl;dr. are you trying to mod your own in your body? cuz thats not possible, atleast with the ODIN kit, i did do the fluorescent protein experiment, but we had to cold shock the bacterium, i highly doubt it would work on human cells, bacterium is easy because when you shock them the plasmid that contains DNA is more accessible. as for getting into the nucleus of a human cells idk.
  • JohnDoeJohnDoe August 2016
    Agreed after the earliest fetal stages with out gene editing nanotech(Semi sarcastic) editing your entire body's genetic make would be a huge challenge. I have also look into this, its not easy.
  • CassoxCassox August 2016
    Yeah. I'm curious about some kind of topical application. Could you simply get the mod into some epithelial cells? I'm sure there would be application for some cells in a small area rather than trying to do a gene mod on an entire organisms cells.
  • BirdhandzBirdhandz August 2016
    I found another company that might have what you are looking for. 

    https://www.horizondiscovery.com/cell-lines/x-man-knockout-cell-lines

    I don't know anything about this sort of thing but would love to play around with glowing bacteria from the Odin kit.  Keeping it below 86 degrees F. would be tough this time of year especially during shipping.  I have sent them an email asking if the heat kills the glowing or if it comes back when it cools down.

    I'm not sure if the glowing bacteria strain could be saved or stored and be revived later or if it is a one time experiment.  With the heat limit, I guess this particular glowing bacteria wouldn't be good for any human applications.

    I'm imagining maybe a living tattoo ink or skin graft that would continue living and glowing feeding on the body itself.  Sounds dangerous if even possible but wouldn't require any batteries or trillium to work.

    Edit to add answers to my own questions.  "One major problem people have is that they let the
    bacteria culture temperature rise above ~80F (30C). While the bacteria
    do just fine at temperatures as high as 98F (37C), the bioluminescent
    genes come from another organism that lives in cooler temperatures. When
    the temperature goes up, the protein catalyzing the bioluminescent
    reaction denature into a nonfunctional shape."

    You can store it in the fridge for a few weeks to keep it alive.  Then you can restreak it onto a new plate to renew the glow.


  • _Larry__Larry_ August 2016
    @Cassox Topical application just gave me an idea! My father has been looking at a specific medication being developed for "vampires" that have a genetic condition with zero medical condition. The drug just causes a lot of melanin production and is temporary. What if we could change the melanin production of the top layer of skin? This would allow control of a very even tan unlike any other chemical. Overall benefits would be reduced skin cancer risk and the ability to fit in on jersey shore!
  • kuroro86kuroro86 August 2016
    Try this start up : 

    http://www.the-odin.com/diy-yeast-crispr-kit/ 
  • WyldstormWyldstorm August 2016
    Hrm.. As i've said before, if i get get a few genetic changes with only <40% risk, ill do it.
    So will look into this again.. 

    That being said, are there highly experienced peeps around here that could/want to help review my changes before i take the plunge?