Genetics and Biology based mods Sat, 22 Jul 17 21:48:27 -0700 Genetics and Biology based mods en-CA Heart rate adjusted by vibration Sat, 22 Jul 2017 02:37:14 -0700 Commander_Keen 2086@/discussions Telomerase Reverse Transcriptase Enzyme Injections for Improving Longetivity Tue, 06 Sep 2016 05:14:52 -0700 Finn333 1720@/discussions Hacks for Heart Disease Tue, 20 Jun 2017 07:32:59 -0700 TheGreyKnight 2056@/discussions @chironex was working on the FIREFLIES system awhile back; In short, "Nanomachines, son." I don't know how far he's developed it yet, but I'd but they'd be great for breaking down arterial plaque. The react series might be able to actively remove "bad" cholesterol from the blood stream too, but I don't know enough about biochemistry yet to know whether it'd be possible or effecient.

Does anyone else have any ideas about how this could be dealt with, beyond the usual "Eat healthy, get enough exercise, and quit smoking"? And are nanomachines even a reasonable answer at this point?]]>
Decellularizing strawberry tissue Wed, 12 Jul 2017 18:19:32 -0700 ThinkerTinkerTrump 2078@/discussions
Last year I came across that thread about decellularizing fruit and vegetable tissue, and have finally worked up the nerve to go through with it. Here are some before and after shots.
I made a couple changes to his methodology: instead of using distilled water, I used purified water which didn't seem to negatively affect anything. I also used a 1% SDS stock solution instead of his 0.5%. This also didn't seem to make any noticeable difference, so it's probably better to stick with a 0.5% concentration to conserve material. Something that wasn't mentioned is how ridiculously fragile strawberry extracellular matrices are. After a certain point I had to avoid splashing the strawberries when changing solution, otherwise they would tear. 

I want to keep this scaffold and a few others I have planned for the future around my dorm as decoration, but once I get the proper space and set up for a cell lab, I'd like to try my hand at reseeding scaffolds as well. Bioluminescent hearts anyone?
Making the human body stronger... by forceing it to become weaker first? Sun, 14 May 2017 06:01:37 -0700 Mael 2025@/discussions

P.s. posting via phone at night so if spelling/details are off that would be why.]]>
Immunofluorescent imaging, nanoparticle systems and preparing NHS fluorescein Mon, 15 May 2017 12:31:49 -0700 chironex 2027@/discussions
Modify your own genetics to have a high proportion of fast twitch muscle? Mon, 03 Apr 2017 02:17:44 -0700 tryingtomakeit 1992@/discussions "These are ACE and ACTN3 genes. Independent research has confirmed the connection between the genotype of ACE (angiotensin converting enzyme) and athletic performance. ACE gene has two gene variants: ACE I and ACE D. Gene variant ACE I raises ability in endurance sports or, in other words, the bearer of this gene has averagely higher endurance. Researches have indicated a more frequent occurrence of this gene combination on many top sportsmen of several endurance sports (running, skiing, rowing, triathlon etc). The bearers of gene variant ACE D have, on the contrary, averagely better abilities of strength and speed." 
 I need ACTN3 and ACE D but if i could change them would they affect my fiber type composition as an adult? are there any other know genes to affect this?

Growing leather using bacteria instead of cows Mon, 01 May 2017 12:12:22 -0700 chironex 2013@/discussions
Biohackers & FDA - Call to Action Wed, 01 Feb 2017 21:51:56 -0800 cyberlass 1922@/discussions At Bodyhacking Con there was discussion about the Josiah Zayner & David Ishee chat on Biohackers and the FDA. In particular the biohacking - DIY Bio community is pushing back against the FDA proposed regulation "Intentionally Altered Genomic DNA in Animals".  This is a starting point for regulation into our community and we should be working together to obtain the best outcome.

Please watch the video: and take action:

If you aren't sure what to write, you can check out the 200+ public comments for ideas

Other things we can do:
  • Raise awareness and support through media 
  • Write a blog post about the conflict the DIY Bio part of our community is experiencing
  • Make/go on a podcast about this issue
  • Interview someone in the DIY Bio community and publish it 
  • Social media posts (I know, I know but it is a factor)
  • Reach out to your DIY Bio contacts and see what they need, let the rest of us know if there is more we can do to help

Augmenting the Sense of Smell Tue, 14 Mar 2017 00:08:59 -0700 Maxwell 1975@/discussions Nerve Enhancement? Tue, 27 Dec 2016 11:15:19 -0800 Cathasach 1885@/discussions
Anyone know what I'm talking about?
Learning microbiology the fun way Mon, 03 Apr 2017 09:58:17 -0700 chironex 1995@/discussions I believe the best way to learn biology, and microbiology in particular, is to experience it. By actually working with the organisms you get so much more understanding than by just reading about them. So to serve as an introduction to microbiology, I made this little tutorial on how to make wine and mead. Yeast is just so easy to cultivate, so it serves as a great teaching tool to get people used to working with organisms like this. I'll be building on it in the future and looking at more advanced skills like using a bioreactor and culturing more difficult organisms. 

Prevent scurvy, increase regeneration of skin and increase overall bodily health through goats Wed, 29 Mar 2017 12:59:28 -0700 Mael 1988@/discussions I recently learned that goats have a gene in their liver that enables them to produce vitamin c independantly and im thinking that perhaps using that gene (which is shown to work in animals) in either bacteria to easily produce vitamin c or, using crispr to edit human cells to produce it might be a good test to run to see if by providing a source of vitamin c creation in the body, one may have the ability to heal collegen and other tissue through there body itself
P.s. posting via phone sorry for poor grammar and inconsitentcy
Radiation Resistant Blood (think The 100) Fri, 10 Mar 2017 12:16:57 -0800 Finn333 1974@/discussions
Firstly, the radiation resistance. I've been reading up on a study that was working on a new way to treat radiation sickness. In the study, they took a sample of e. coli and subjected it to radiation. After 99 percent of the sample died, they let the population grow back. After twenty iterations of rinse and repeat, the team created a sample of e. coli that could reverse the effects of radiation. Then, you take a sample of your blood. You then bind the e. coli to the sample and inject it into yourself. Now, you have a bigger radiation tolerance than most people.
Stimulating Muscle Growth Thu, 23 Mar 2017 19:46:38 -0700 Liamwils404 1984@/discussions]]> TUES FEB 21 IS THE NEW WEBSITE GO-LIVE [CROSS-CATEGORY POST] Fri, 17 Feb 2017 13:24:54 -0800 cyberlass 1945@/discussions

Note: Your user account will be on new system, BUT you will have to do a password reset.
Homo sapien mutagensis Wed, 18 Jan 2017 14:11:13 -0800 InDubiousBattle 1909@/discussions Been a troll for a while and this is my first post

Wanted to start to share some of the stuff I have been working on in regards to human genetic design. Sorry there is an external link but it happens.
I have tons of information so it will take a while to have it internet ready so I hope people can be patient. I also want to tell a good story.

Anyways, hope this interests people here.

Hive Body Modification Mon, 03 Oct 2016 11:10:18 -0700 kscottb 1757@/discussions personally i would think creating a sub-dermal/trans-dermal hive cavity on a shoulder would by the best way to do this, I was wondering also if one was capable of creating this "hive-space" if one could modify there body to create a hormone that attracts a said insect to the body, calling it their home and if possible a hormone of danger(Similar to a Queen releasing a hormone that the hive is under attack)  or if not produced from the body organically possible artificially? I think it would be more of a interesting project in term of symbiosis and modification of the body required. im not so much concerned of they "why would you do that", but rather the if its possible, how would it be done?]]> The mysterious 98%: Scientists look to shine light on the 'dark genome' Sat, 04 Feb 2017 05:38:15 -0800 SourcePhoenix 1926@/discussions

I think it's mainly a very complex translation process for 1) useful functions, 2) frameworks for patterns, geometry, compression, etc. 3) shared abstract memories shared at the species level or a sub scope of some sort 4) check systems 5) a merging system that's specifically adapted for the potential of mutations and maybe tries to harness "mutational noise" 6) ... I could think about this more but I got work to do.

What do you all think?

I wonder if A.I. systems & genetic algorithms incorporate this today.
Real & theorical symbiosis usage in bio/gene modifcation Tue, 07 Feb 2017 17:01:04 -0800 SymbiosisIsKey 1931@/discussions media take off in many ways since the 1940s sci-fi boom. Whether it be the idea of alien creatures
from other worlds bonding with people(The Venom family of Symboties, Guyver Units and so on). Or plants, parasites and biomedical made
creatures hybridized with humans to something more(Metal Gear's The End, Parasyte). But we also have rather real
possibilities for symbiosis, as shown by microbiota, already available in the real world. For those unaware we
have an ecosystem of things living within or on us that make our life generally
easier and we are keeping them fed, sheltered and safe for lack of a better analogy. As my name and the title suggest, I believe deep down bio and gene modding can be improved if we push elements of symbiosis further, we'd just need to bio and gene mod other creatures as well as ourselves. Imagine if we could improve the proteins a medical leech(Hirudo Medicinalis) produces to increase better blood flow through out more of the body while still feeding it. Or taking any of the many elements of helminethic therapy (using what is normally considered parasitic creatures as helpers to tackle issues) and modifying their genetics to improve other things within us; namely to the point they become mutually beneficial to us and no longer a hazard.

Have you had thoughts of this? Do you know anyone actually trying to do this?  Ever catch a glimpse of someone possibly on to something and want to share? I've read a few topics on here that dip into on this subject and wanted to unify it with one topic and get people who have had it on their minds.
PCR Heat-Block Tue, 27 Dec 2016 19:29:35 -0800 TorrentX 1886@/discussions
1.) Am I missing something?  Is there a place to purchase cheap heat-blocks for .2ml eppendorf tubes?  Or an alternative solution.
2.) What have others that ran into the problem done? Just ponied up for the expensive heatblock or tinkered with a homemade one til it worked?
3.) If there's no good answer to the first two questions, is this something that the community would find valuable if I worked on a solution for?
Cellular Matrix Tue, 03 Jan 2017 10:12:50 -0800 SourcePhoenix 1893@/discussions A list of links on tight junctions for cell adhesion(WIKIPEDIA) Thu, 05 Jan 2017 08:17:38 -0800 Zwytechhacker 1897@/discussions
Decellularizing tissue Sun, 13 Mar 2016 21:29:03 -0700 chironex 1396@/discussions
Genetic Modification of Yeast and Bacteria to work for us Sat, 24 Dec 2016 09:35:22 -0800 RealityWizard 1882@/discussions Ce6 Tue, 25 Nov 2014 12:33:48 -0800 glims 745@/discussions
We should have them ready soon. Basically, I'm just making sure that I can get the amounts right and not scorch your eyes. The papers and patents we have access to are very specific in some regards and very vague in others. As @Cassox mentioned, this chemical is used in lazering cancer cells (my paraphrase) so obviously, exploding your eye bits by tiny molecular focuses of daylight would... suck.

Progress is being made. It's just me reading and then mixing things together and measuring light bits.

For those who are ready to chip in and talk about that patent, their amounts are theoretical and were used just to lock the patent down. Got get this right so nobody gets hurt. Eyes are important

Anyone have any questions, let me know.
Removing Toenails Thu, 01 Sep 2016 18:04:17 -0700 eggit 1711@/discussions dna & coding.. Tue, 22 Nov 2016 06:12:45 -0800 Wyldstorm 1832@/discussions And one thing i cant really find good data on is level 2+ coding.. 

So, anyone know?
How many levels of coding are there in the dna?
How the hell do i compute the consequences?
Is there a good refutation?
New Crispr News!! Sat, 19 Nov 2016 14:54:00 -0800 _Larry_ 1824@/discussions China just made some rather large advances with real life uses for Crispr!

Has anyone shot yourself with Capsaicin? Fri, 22 Jul 2016 11:27:48 -0700 opi 1646@/discussions
I'm interested in its effect on systemic pain nerve degeneration to become a pain-free-forever cyborg, but I also heard it can give you a sense of "high". So yeah, for recreational or whatever other purposes, I'd like to hear your adventures with Capsaicin! :)

Some stuff I think I know about Capsaicin:
- desensitize TRPV1 nerves by destroying them
- better to inject intrathetically to minimize systemic side-effects while killing main spinal pain nerves
- systemic temporary side effects include bradycardia, apnea, body temp decrease

Since I can't inject in my own spine, the most practical way seems to be into a vein.
My hypothesis is that if I inject very little each time, it will affect the heart and respiratory functions minimally, and some Capsaicin will end up in the CSF, killing a few pain nerves there, and if I repeat this many times, I can eventually get rid of most pain nerves.